The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both the male and female germline. As the techniques used for isolating and studying male and female germ cells are slightly dissimilar, the different technologies are grouped under two sections in this volume, namely, male germline and female germline. This collection brings the field of germline developmental studies together such that readers can find, in a single volume, techniques that allow the isolation of gonads with germ cells at different stages of development, the study of the genomics and epigenomics of germ cells, and an examination of the role of single genes in the developmental potential and function of germ cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and the avoidance of known pitfalls. Comprehensive and cutting-edge, Germline Development: Methods and Protocols serves as a comprehensive text to individuals, particularly researchers in reproductive and developmental biology, who are interested in studying cells of the germ lineage.

Jane Moore busts open the stuff we rely on when it comes to waging domestic germ warfare. Jane has her own home scrutinized for germs in order to put antibacterial spray to the test, finds out how some mind-blowingly clever molecules in air freshener manage to combat dirt in mid air, and discovers the best wonderstuff on offer for the dirtiest of all household jobs - cleaning the loo.

Plot: Set in Victorian London, Tanya Wexlera€™s period comedy Hysteria tells the tale of Mortimer Granville (Hugh Dancy), a young doctor disturbed by the way most hospitals ignore the latest research on germ theory, and still treat patients with bleedings and leeches.

Jane Moore busts open the stuff we rely on when it comes to waging domestic germ warfare. Jane has her own home scrutinized for germs in order to put antibacterial spray to the test, finds out how some mind-blowingly clever molecules in air freshener manage to combat dirt in mid air, and discovers the best wonderstuff on offer for the dirtiest of all household jobs - cleaning the loo.

Its the height of the Cold War and the United States government is desperate to combat the spread of Communism. The CIA launches a highly classified, top secret research program into the covert use of biological and chemical agents. In simulated attacks on enemy populations, entire cities in America are contaminated with bacteria, exposing millions of Americans to germ warfare. But the real focus of the research is on mastering the art of mind control.

Set in Victorian London, Tanya Wexler's period comedy Hysteria tells the tale of Mortimer Granville [Hugh Dancy), a young doctor disturbed by the way most hospitals ignore the latest research on germ theory, and still treat patients with bleedings and leeches.

It's the height of the Cold War and the United States government is desperate to combat the spread of Communism. The CIA launches a highly classified, top secret research program into the covert use of biological and chemical agents. In simulated attacks on enemy populations, entire cities in America are contaminated with bacteria, exposing millions of Americans to germ warfare. But the real focus of the research is on mastering the art of mind control. Psychiatrists at top academic institutions work under secret contract with the agency. Psychiatric patients, prisoners, even unwitting members of the public are exposed to a startling array of experiments designed to facilitate interrogations, induce amnesia and program in new behavior. Every psychological technique is explored, including hypnosis, electroshock therapy and lethal cocktails of drugs. What was the extent of these brainwashing experiments...

It's the height of the Cold War and the United States government is desperate to combat the spread of Communism. The CIA launches a highly classified, top secret research program into the covert use of biological and chemical agents. In simulated attacks on enemy populations, entire cities in America are contaminated with bacteria, exposing millions of Americans to germ warfare. But the real focus of the research is on mastering the art of mind control. Psychiatrists at top academic institutions work under secret contract with the agency. Psychiatric patients, prisoners, even unwitting members of the public are exposed to a startling array of experiments designed to facilitate interrogations, induce amnesia and program in new behavior. Every psychological technique is explored, including hypnosis, electroshock therapy and lethal cocktails of drugs. What was the extent of these brainwashing experiments...

Was the disease known as the Black Death, which killed one-third of Europes population in just 3 years during the Middle Ages and the bubonic plague the same disease? What could cause the spread of such a horrendous plague? In 1347 A.D, a Mongol army attacked Kaffa, an ex-patriot Genoese city located in the Crimea. The Mongols employed a crude form of germ warfare by catapulting bodies of their own men, dead from a mysterious disease, over the fortification wall. It turned out that they were infected by "the black death". Soon the forts Genoese defenders were infected. Some of the Genoese sailors managed to escape and sail back to their homeland, but they took with them an unwelcome stowaway, a black rat that spread the disease at every port.

It's the height of the Cold War and the United States government is desperate to combat the spread of Communism. The CIA launches a highly classified, top secret research program into the covert use of biological and chemical agents. In simulated attacks on enemy populations, entire cities in America are contaminated with bacteria, exposing millions of Americans to germ warfare. But the real focus of the research is on mastering the art of mind control. Psychiatrists at top academic institutions work under secret contract with the agency. Psychiatric patients, prisoners, even unwitting members of the public are exposed to a startling array of experiments designed to facilitate interrogations, induce amnesia and program in new behavior. Every psychological technique is explored, including hypnosis, electroshock therapy and lethal cocktails of drugs. What was the extent of these brainwashing experiments...

It's the height of the Cold War and the United States government is desperate to combat the spread of Communism. The CIA launches a highly classified, top secret research program into the covert use of biological and chemical agents. In simulated attacks on enemy populations, entire cities in America are contaminated with bacteria, exposing millions of Americans to germ warfare. But the real focus of the research is on mastering the art of mind control. Psychiatrists at top academic institutions work under secret contract with the agency. Psychiatric patients, prisoners, even unwitting members of the public are exposed to a startling array of experiments designed to facilitate interrogations, induce amnesia and program in new behavior. Every psychological technique is explored, including hypnosis, electroshock therapy and lethal cocktails of drugs. What was the extent of these brainwashing experiments...

It's the height of the Cold War and the United States government is desperate to combat the spread of Communism. The CIA launches a highly classified, top secret research program into the covert use of biological and chemical agents. In simulated attacks on enemy populations, entire cities in America are contaminated with bacteria, exposing millions of Americans to germ warfare. But the real focus of the research is on mastering the art of mind control. Psychiatrists at top academic institutions work under secret contract with the agency. Psychiatric patients, prisoners, even unwitting members of the public are exposed to a startling array of experiments designed to facilitate interrogations, induce amnesia and program in new behavior. Every psychological technique is explored, including hypnosis, electroshock therapy and lethal cocktails of drugs. What was the extent of these brainwashing experiments...

Was the disease known as the Black Death, which killed one-third of Europe's population in just 3 years during the Middle Ages and the bubonic plague the same disease? What could cause the spread of such a horrendous plague? In 1347 A.D, a Mongol army attacked Kaffa, an ex-patriot Genoese city located in the Crimea. The Mongols employed a crude form of germ warfare by catapulting bodies of their own men, dead from a mysterious disease, over the fortification wall. It turned out that they were infected by "the black death". Soon the fort's Genoese defenders were infected. Some of the Genoese sailors managed to escape and sail back to their homeland, but they took with them an unwelcome stowaway, a black rat that spread the disease at every port.

Was the disease known as the Black Death, which killed one-third of Europe's population in just 3 years during the Middle Ages and the bubonic plague the same disease? What could cause the spread of such a horrendous plague? In 1347 A.D, a Mongol army attacked Kaffa, an ex-patriot Genoese city located in the Crimea. The Mongols employed a crude form of germ warfare by catapulting bodies of their own men, dead from a mysterious disease, over the fortification wall. It turned out that they were infected by "the black death". Soon the fort's Genoese defenders were infected. Some of the Genoese sailors managed to escape and sail back to their homeland, but they took with them an unwelcome stowaway, a black rat that spread the disease at every port.

..the vast numbers of books devoted to the subject...have done little to improve the situation...Andrew and Mary Bragg's Developing New Business Ideas might just be the book to change much of this...If this book can help investors and would-be entrepreneurs in this area it will do not just them, but also the economy as a whole a great service." Roger Trapp, Independent on Sunday "Developing New Business Ideasis a truly original book, with a fabulous blend of lessons and inspiring real business stories. Most importantly, it demonstrates in a practical and easy to understand manner how to put creativity and innovation into action." - Lord Bilimoria CBE DL, founder and Chief Executive, Cobra Beer At some time in their life, almost everyone in business has an idea for a new enterprise. The idea might be sparked by dissatisfaction with an existing product, experiencing something new while traveling or simply finding that nobody in the market is serving your particular need. The idea could be for a new venture for your business to launch, or for new business of your own. But most ideas remain just that: ideas.We struggle to develop them, wondering if they will work, why nobody has already thought of them and how we could ever turn them into a profitable reality. How do you take a promising thought and develop it into an enterprise worth backing? That is the question that this book sets out to answer. In Developing New Business Ideas, Andrew and Mary Bragg provide a practical guide to creating, shaping, evaluating, refining and implementing new business ideas. The book demonstrates that it is within absolutely everybody's power to take the first germ of a business idea and to systematically creatively challenge and commercially develop that idea into a viable business opportunity. Using a practical four-step creative process, Developing New Business Ideas offers a wide-range of hands-on, how-to-actually-do-it techniques to help you add value and viability to your original idea. It will also introduce you to the experiences of a range of today's successful entrepreneurs, enabling you to learn from the creative lessons that they learned as they built their businesses.Supported by its own set of online tools, Developing New Business Ideas is an imaginative and interactive companion to converting creativity into successful enterprise. "Having had your bright, fresh, original idea, the really hard part is turning it into successful reality. By detailing four structured steps to developing saleable products or services from that first idea, this excellent and imaginative guide significantly simplifies that task. Essential reading for all would-be entrepreneurs". Trevor Baylis OBE, the inventor of the clockwork radio."